Summary
Type: SPE
Application #: 300640
Catalog#: 730141 - SPE Cartridges, Chromabond, C18 ec, 6ml, PP, 2000mg 30/pk
Detection:
Conditions: Column conditioning:
15 ml methanol, then 15 ml dist. water
Sample application:
Slowly pour 15 ml nutrient solution through the column
Column washing:
20 ml water; dry column in a stream of air (e.g. water jet pump) for about 30
min
Elution:
20 ml methylene chloride, elute slowly and evaporate
For further analysis we recommend a HPLC column ET 250/4 NUCLEOSIL® 120-5 C18,
mobile phase acetonitrile – water (40 : 60, v/v), 1.0 ml/min, UV detection at
248 nm
Authors: Macherey-Nagel
Source: LC department